γH2AX Assay as DNA Damage Biomarker for Human Population Studies: Defining Experimental Conditions
نویسندگان
چکیده
منابع مشابه
The γH2AX DNA damage assay from a drop of blood
DNA double-strand breaks (DSB) and blocked replication forks activate the DNA damage response (DDR), a signaling pathway marked by phosphorylation of histone 2AX (H2AX). The phosphorylated form, γH2AX, accumulates at the site of damage and can be detected as foci by immunocytochemistry. Therefore, γH2AX is a sensitive and robust biomarker of DNA damage, notably DSB. Cells from peripheral blood ...
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ژورنال
عنوان ژورنال: Toxicological Sciences
سال: 2015
ISSN: 1096-0929,1096-6080
DOI: 10.1093/toxsci/kfv011